We are exploring the clinical utility of high-resolution cone photoreceptor imaging device: rtx AO camera. Through this device, individual cone cells can be visualised and counted throughout the macular region (central 20° × 20° area of the retina). Each image frame covers 4° × 4° area of the retina. We have a large AO image library of healthy controls and patients with retinal diseases (colour vision deficit, laser injury, drug toxicity, inflammatory eye disease, inherited retinal disease and macular degeneration). Undergraduate and postgraduate students and junior doctors can be involved in developing new methods of images processing and analysis. Variables such as cone density and Nyquist frequency are measured at retinal loci determined by cartesian and polar coordinate systems.